Piping and Control Unit
The fermenter is provided with a piping and control system enclosed in a stainless steel, floor standing cabinet or mounted in an open frame.
In the closed cabinet type the lower part of the cabinet contains valves, circulation pump, heater etc. to conduct gas, steam and water for sparging, sterilisation and temperature control during the process.
The gas flow can be set with a manually operated valve and is monitored by a mechanical flow meter. The system may optionally include electronic flow measurement of the gas flow and regulation through the Novaferm computer control system by sensor and control valve in the gas inlet line.
Sterilisation, cooling and fermentation temperatures are controlled by solenoid and pneumatically operated valves, circulation pump and heater governed from the computer control system. Manometers at the front indicate the pressure of the connected media.
The electric cabinet is placed at the upper part of the piping system and contains the electric components, the Novaferm Fermenter Control System and additional instrumentation. The cabinet is provided with fan for forced ventilation.
All connections of supply media are placed at the rear of the bottom frame.
Control Unit
The system is provided with the Novaferm Fermenter Control System for direct digital control of fermentation processes based on programmable logical controllers from the SIMATIC S7 (Siemens) family. The modular concept allows control of fermenter systems from laboratory to industrial scale.
The standard control system includes hard- and software for control of temperature, speed and pH and measurement of dissolved oxygen. As an option dissolved oxygen can be cascade controlled by agitator speed and air flow. Additional parameters can be supplied on request. PC hardware is not included in the standard package.
Sensor Kit
The standard kit contains the following:
- 2 pcs Pt100 sensors with cables and connectors
- 1 pce Inductive sensor with cable and connector
- 1 pce pH sensor (Mettler) with cable and connector
- 1 pce pO2 sensor (Mettler) with cable and connector
- 1 pce Foam sensor with cable and connector
Additional sensors on request.
Pump Unit
Peristaltic pump for dosing of acid, alkali or other addition. Fix speed pump. Including glass container, tubing, sterile filter and needles. Container, hose and needles to be sterilised in autoclave. Delivered with silicone hose diameter 6,4/3,2 mm.
Technical Specification, Fermenter Vessel, 420 L
Vessel
Material: Acid resistant stainless steel equal to AISI 316L
Working pressure: 3 bar overpressure and full vacuum
Conditioning, vessel interior: Ground, grit 220, polished
Conditioning, vessel exterior: Ground, grit 180, polished
Bottom: Dished, with agitator
Jacket
Material: Acid resistant stainless steel equal to AISI 316L
Working pressure: 3 bar overpressure and full vacuum
Lid
Material: Acid resistant stainless steel equal to AISI 316L
Conditioning: Ground, grit 220, polished
Dished, diam. 450 mm, with six clamps.
Stand
Material: Stainless steel
Conditioning: Ground, grit 180, brushed
On wheels, with tilting possibility.
Agitator
Bottom mounted, magnetically coupled. Torque 48 Nm, power 4,5 kW, speed 0-1000 rpm. Impeller, flat turbine, 6 blades. Other powers on request. Tungsten carbide bearings.
Seals
Material, packings and seals: EPDM
Connections
Connections in lid: Sight glass, diam. 80 mm
Connections in top: 8 pcs E-connections, diam. 25 mm
Connections in side, upper level: Gas inlet, gas outlet
Connections in side, lower level: 5 pcs E-connections, diam. 25 mm
Connections in bottom: 1 pce temp. sensor pocket, diam. 6 mm
Bottom valve for aseptic drain
Connections in jacket: TC-clamp flanges
Sparger
Material: Acid resistant stainless steel equal to AISI 316L
Single nozzle below impeller. Multi nozzle, ring type etc. on request.
Baffles
Material: Acid resistant stainless steel equal to AISI 316L
With 4 baffle plates. Detachable.
Pt 100 pocket
Material: Acid resistant stainless steel AISI 316L for Pt 100 sensor
Sterile filter
Heated filter housing. Capacity 600 l/min, incl. filter cartridge glass fibre deep filter type, or 0.22 µm hydrophobic PTFE filter cartridge.
Accessories
Sampler in acid resistant stainless steel equal to AISI 316L. In situ sterilisable
Rubber septum connector with 7 holes
Stainless steel wire reinforced PTFE-hoses for connection of jacket to steam/condensate and water/drain, aeration and filter jacket to steam and drain
Sight glass illumination
Seal kit
Tool kit
Volume
Total volume 420 l, working volume approx. 300 l
Dimensions
H=2000 mm, W=850 mm, L=1100 mm
Vessel diam.=650 mm
Weight, approx. 350 kg
Design
According to the Swedish Code for pressure vessels
Technical Specification
Piping and Control Cabinet for Fermenters 420 L
MATERIAL
Cabinet: Stainless steel
Tubing: Stainless steel
Components: Stainless steel, brass/gunmetal
DESIGN
As per Swedish regulations for electrical installations. Electrical parts splash protected, mainly IP54.
CONNECTION DATA
AUX. AIR
Conn. No. 15
Max. consumption 5 l/min free air
PROCESS GAS IN
Conn. No. 20
Max. consumption 600l/min free gas
WATER
Conn. No. 20
Max. consumption 50 l/min
STEAM
Conn. No. 20
Rec. min capacity 150 kg/h
DRAIN
Conn. No. 25
Max. capacity 60 l/min
ELECTRICITY
Voltage (see below)
Max. consumption 10 Kw
Gas filter, in
Conn. No 20
Gas filter, out
Conn. No. 20
Vessel jacket, up
Conn. No. 20
Vessel jacket, down
Conn. No. 20
All connections for inlet supplies media are provided with female pipe thread type BSP.
MEDIA REQUIREMENTS
AUXILIARY AIR
Pressure 4-6 bar
Dry, particle free
PROCESS GAS
Pressure 2 bar
Dry, oil free, particle free
WATER
Pressure 2-4 bar
Particle free, max. temp. 5o below min. vessel control temp
STEAM
Pressure 2 bar
Dry, saturated, particle free
DRAIN
Free flow
ELECTRICITY
Voltage 3x380V+N+G, 50 Hz
Transient free, voltage stability +-5%
DIMENSIONS
Height=2000 mm, Width=630 mm, Depth=820 mm
Weight, approx. 200 kg
